A nanobody inhibitor of Fascin-1 actin-bundling activity and filopodia formation

Fascin-1-mediated actin-bundling activity is central to the generation of plasma membrane protrusions required for cell migration. Dysregulated formation of cellular protrusions is observed in metastatic cancers, where they are required for increased invasiveness, and is often correlated with increased Fascin-1 abundance. Therefore, there is interest in generating therapeutic Fascin-1 inhibitors. We present the identification of Nb 3E11, a nanobody inhibitor of Fascin-1 actin-bundling activity and filopodia formation. The crystal structure of the Fascin-1/Nb 3E11 complex reveals the structural mechanism of inhibition. Nb 3E11 occludes an actin-binding site on the third β-trefoil domain of Fascin-1 that is currently not targeted by chemical inhibitors. Binding of Nb 3E11 to Fascin-1 induces a conformational change in the adjacent domains to stabilize Fascin-1 in an inhibitory state similar to that adopted in the presence of small-molecule inhibitors. Nb 3E11 could be used as a tool inhibitor molecule to aid in the development of Fascin-1 targeted therapeutics.


Introduction
Fascin-1 is an actin-binding protein that promotes the assembly of actin filaments into parallel bundles.Fascin-1-dependent bundling of F-actin underlies the formation of several classes of plasma membrane protrusions that drive cell migration, including finger-like filopodia and broad, flat lamellipodia that advance the leading edge of a migrating cell [1][2][3][4].Fascin-1 is also involved in the formation and dynamics of podosomes and invadopodia which facilitate cellular invasion of the extracellular matrix [5][6][7].Fascin-1 is a 55 kDa globular protein composed of four tandem β-trefoil domains that pack together to form a compact structure approximately 6 nm in diameter.It has at least two actinbinding surfaces and in filopodia, Fascin-1 cross-links parallel actin filaments into a hexagonal lattice, forming rigid bundles approximately 140 nm in diameter [8][9][10][11][12].Phosphorylation of Fascin-1 at Ser 39 by protein kinase C (PKC) negatively regulates its actin-binding activity and has been suggested to contribute to the highly dynamic nature of filopodial bundles [13][14][15].Actin-based plasma membrane protrusions are crucial in embryonic development, for the function of motile cell types such as macrophages, and in processes such as wound healing [1][2][3].However, in tumour cells, these structures enable metastasis and are frequently dysregulated in metastatic cancer, with greater size, longevity and number of protrusions associated with increased invasiveness.Fascin-1 expression, which is undetectable in normal adult epithelia [16], is upregulated in multiple cancer types and is often correlated with metastatic spread and poor patient outcome [17][18][19][20].Fascin-1 is therefore of interest as a prognostic indicator and a therapeutic target.Small-molecule inhibitors of Fascin-1 are in development and have been used to target cancer cell invasion in vitro and in model systems [10,[21][22][23][24][25][26][27].The first Fascin-1 inhibitor to reach clinical trials was NP-G2-044.This compound appeared to exhibit some anti-tumour and anti-metastatic activity in a Phase 1 trial, although these improvements were not significant according to RECIST guidelines [28].Such developments confirm that inhibition of Fascin-1 is a promising avenue and that more research is needed to find new ways to target Fascin-1 with greater efficacy.
Heavy chain only antibodies (HcAbs), which are produced by camelids and cartilaginous fish, lack light chains and bind antigens through a single variable domain on each heavy chain, known in camelids as VHH domains [29].Within VHH domains, antigen binding primarily occurs through three variable complementarity determining regions (CDRs), which are separated by four constant framework regions (FRs).VHH domains can be isolated from HcAbs to derive single-domain antibodies (sdAbs).sdAbs are stable, monomeric protein reagents of approximately 15 kDa in size that can be produced in bacterial expression systems.Because in vitro screening methods can be used to isolate examples that have high affinity and specificity for an antigen of interest and any other properties that are required, camelid sdAbs, also known as nanobodies (Nbs), are increasingly being exploited in multiple applications, such as markers in high-resolution microscopy studies [30][31][32], in the development of biosensors [33], in medical imaging with potential for conjugation to radionuclides/photodynamic agents for targeted therapy [34,35], and as crystallization chaperones to stabilize conformationally flexible proteins [36][37][38].Furthermore, there is considerable interest in the therapeutic potential of Nbs as inhibitors of the activities and interactions of target proteins, particularly because Nbs can interact with cryptic, shallow epitopes on antigens that are difficult to target by traditional small-molecule drug discovery methods [39][40][41][42].Indeed, caplacizumab is the first Nb approved for clinical use to treat acquired thrombotic thrombotcytopenic purpura [43].
Here, we have sought Nb inhibitors of Fascin-1 and report the identification of Fascin-1-specific Nbs by phage display biopanning and the isolation of Nb 3E11 which acts as a potent inhibitor of Fascin-1 actin-bundling activity.A crystal structure of Nb 3E11 in complex with Fascin-1, combined with mass spectrometry methods, demonstrates the mechanism of Fascin-1 inhibition by Nb 3E11, which is distinct from that of current small-molecule inhibitors, providing new insight into how Fascin-1 inhibition can be achieved.

Isolation of Fascin-1-specific nanobodies
Two llama nanobody phagemid libraries were generated using the peripheral blood mononuclear cell (PBMC) RNA from animals immunized with purified recombinant human Fascin-1 1-493.DNA sequencing of random clones from the libraries confirmed the presence of functional VHH coding sequences in greater than 80% of phagemid constructs.The total number of clones in each library was 1.17 × 10 9 for library 1 and 1.22 × 10 9 for library 2.
Three rounds of phage display biopanning were carried out with increasing stringency to isolate high affinity Fascin-1 nanobodies.Enrichment over the negative control ( pan with no Fascin-1 immobilized) was observed (electronic supplementary material, table S1).Forty-four positive clones for each library pan output were randomly selected and used to express phage-nanobodies in E. coli TG1 and extracted by osmotic shock.The periplasm extracts were used as the input for streptavidin-based ELISAs using immobilized biotinylated Fascin-1.Fascin-1-specific nanobodies were identified as clones for which binding was observed to Fascin-1-coated wells and not the negative control reaction, where no Fascin-1 was immobilized (results for Pan Round 3 are shown in electronic supplementary material, figure S1a).One hundred and ninety-six positive clones from both libraries were sequenced and classified into 12 families based upon sequence identity in the CDRs (figure 1a).The phage-nanobody ELISA results for representatives of each sequence family are shown in figure 1b.

Biochemical characterization of Fascin-1-specific nanobodies
The selected representatives of each sequence family were recombinantly expressed as C-terminally His-tagged Nb proteins in the periplasm of E. coli SS320 and purified to homogeneity.Size-exclusion chromatography (SEC) revealed that Nbs 3B9 and 3G9 each displayed two populations (electronic supplementary material, figure S1b) corresponding to dimer and monomer protein.These two sub-populations were kept separate for further characterization.
In vitro co-precipitation assays with purified Nbs confirmed that all Nbs bound to Fascin-1 (figure 1c).Actin-bundling cosedimentation assays revealed that 13 of the 14 Nbs did not negatively modulate the actin-bundling activity of Fascin-1 (figure 1d and electronic supplementary material, figure S1c-h).However, inclusion of Nb 3E11 resulted in a marked shift in Fascin-1 and actin distribution from the polymerized actin pellet fraction into the supernatant, indicating inhibition of Fascin-1mediated actin-bundling by Nb 3E11 (figure 1e).

Nb 3E11 exhibits dose-dependent inhibition of Fascin-1-mediated actin-bundling
Titration of the Nb 3E11 : Fascin-1 molar concentration ratio in co-sedimentation assays demonstrated that Nb 3E11 acted as a dose-dependent inhibitor of Fascin-1 actin-bundling activity, achieving 100% inhibition at an equimolar concentration.In addition, Nb 3E11 at high concentrations shifted into the supernatant fraction together with actin and Fascin-1 (figure 1f,g).
To ensure that the CDR sequences of Nb 3E11 were responsible for the inhibitory action of the protein, control Nbs were constructed in which the CDRs were exchanged for unrelated CDRs (taken from Nbs specific to bacterial thermophilic proteins) royalsocietypublishing.org/journal/rsob Open Biol.14: 230376 while retaining the constant FRs of Nb 3E11.Two different control Nbs did not show observable binding to Fascin-1 in co-precipitation assays (electronic supplementary material, figure S2a), indicating the specific role of the Nb 3E11 CDR sequences.When these constructs were subsequently used as negative controls in actin-bundling assays and experiments in cells, they did not affect Fascin-1 actin-bundling activity (figure 1g and electronic supplementary material, figure S2b,c).royalsocietypublishing.org/journal/rsob Open Biol.14: 230376

Structural basis of the Fascin-1/Nb 3E11 interaction
A structure of the Fascin-1 1-493/Nb 3E11 complex was resolved by X-ray crystallography at 2.2 Å resolution (table 1 and figure 2a).There are four copies of a 1 : 1 complex of Fascin-1/Nb 3E11 in the asymmetric unit (ASU) (electronic supplementary material, figure S3a).The other three complexes in the ASU display an overall Cα RMSD of 0.19-0.27Å when compared to the chain A/B complex (electronic supplementary material, figure S3b).Unambiguous electron density was observed for all chains (electronic supplementary material, figure S3c).Fascin-1 displays the four tandem β-trefoil folds observed in other structures [8,21].Nb 3E11 adopts an immunoglobulin fold with a single disulfide bridge present and interacts directly with β-trefoils 2, 3 and 4 of Fascin-1 (figure 2a).
An average surface area of 841 Å 2 per molecule is buried at the interface between Fascin-1 and Nb 3E11 (electronic supplementary material, figure S3d).All three CDRs and FR3 of Nb 3E11 form key interactions with Fascin-1 (figure 2b).From CDR1, the side-chains of Arg 29 and Ser 32 form hydrogen bonds with Fascin-1 β-trefoil 4 residues Ser 428 and Leu 416 , respectively; an additional hydrogen bond is present between Ser 33 of Nb 3E11 and Ala 191 of β-trefoil 2; and the aromatic side-chain of Phe 31 is stacked against His 193 .From CDR2, Thr 57 and Thr 59 form a hydrogen bond network involving Lys 379 and Glu 288 of β-trefoil 3 and the backbone of Gly 56 forms a hydrogen bond with Phe 418 of Fascin-1 β-trefoil 4. From CDR3, residues Tyr 104 and Tyr 108 hydrogen bond with Asp 192 of β-trefoil 2 and Glu 288 of β-trefoil 3, respectively.The constant region of Nb 3E11 also contributes directly to the interaction through residues Arg 74 and Asn 76 of FR3, which are central to a hydrogen bonding network involving Glu 417 , Phe 418 , Asn 424 and Tyr 432 of β-trefoil 4 (figure 2b).royalsocietypublishing.org/journal/rsob Open Biol.14: 230376 A binding affinity of 25 ± 0.9 nM between Nb 3E11 and Fascin-1 1-493 was measured by enzyme-linked immunosorbent assay (ELISA) (figure 3a).The strong binary interaction allowed hydrogen-deuterium exchange mass spectrometry (HDX-MS) to be used to validate the complex crystal structure by probing the changes in protein solvation associated with complex formation in solution.The sequence coverage of Fascin-1 was 95% and 90.2% for Nb 3E11 (figure 3b).Reduction in deuterium uptake   royalsocietypublishing.org/journal/rsob Open Biol.14: 230376 upon complex formation represents increased protection and/or reduced solution dynamics of the residues involved and indicates association with protein-protein binding interfaces.Fascin-1 β-trefoil 2 peptide 168 DVPWGVDSL 176 and β-trefoil 4 peptide 415 QLEFNDGAYNIK 426 , which overlap with the binding interface identified in the complex crystal structure, displayed reduced deuterium exchange in the presence of Nb 3E11.Similarly, Nb 3E11 peptides corresponding to CDR1 27 SGRIFSSTRMGW 38 , CDR2 54 VWGTTTTY 61 , CDR3 110 AFNPDYWGQGTQ 121 and FR3 72 ISRDNAKNMVF 82 all displayed a reduction in deuterium exchange upon interaction with Fascin-1, which correlated with the interface observed in the crystal structure (figure 2b and electronic supplementary material, figure S3d).
Residues displaying a substantial increase in deuterium uptake upon complex formation, implying increased solvent exposure, likely resulted from conformational changes induced in these regions concomitant with binding.Positive shifts in deuterium exchange rate upon complex formation were observed for Fascin-1 β-trefoil 4 peptide 398 RKVTGTLDANRSSYDVF 414 , which is adjacent to the binding site identified by HDX-MS analysis and in the crystal structure, and Nb 3E11 CDR3 peptide 103 YYSG-TYL 109 , which is involved in direct binding to Fascin-1 but is only partially buried in the crystal structure (figure 2b and electronic supplementary material, figure S3d).
The strong correlation between the binding interfaces identified by HDX-MS analysis and those in the Fascin-1/Nb 3E11 crystal structure serve to validate the structural basis of the interaction observed by X-ray crystallography, providing a foundation on which to understand how Nb 3E11 inhibits Fascin-1 activity.

Mechanism of Fascin-1 inhibition by Nb 3E11
In previous work, mutagenesis of surface residues has been used to identify two conserved actin-binding sites (ABS) on Fascin-1.ABS1 and ABS2 are surface patches formed by coordination of β-trefoil 1 with β-trefoil 4 and β-trefoil 2, respectively.Mutations on β-trefoil 3, including K379A, also disrupt actin-bundling activity and have been suggested to fall within either a third ABS or an extension of ABS1 or ABS2 (figure 3c) [9].
Nb 3E11 acts to inhibit Fascin-1-mediated actin-bundling activity at multiple ABS.Fascin-1 proteins mutated within ABS1 or ABS3, and that exhibit a greater than 80% reduction in actin-bundling activity compared to WT Fascin-1, were further inhibited by addition of Nb 3E11 [9] (electronic supplementary material, figure S4a).The inhibitory activity of Nb 3E11 at ABS3 likely results from direct occlusion of Lys 379 on β-trefoil 3 (figures 2b and 3c).The Fascin-1 K379A mutant retained the ability to bind Nb 3E11 (electronic supplementary material, figure S4b), which is unsurprising given the extensive buried surface area at the interface (electronic supplementary material, figure S3d).
Nb 3E11 also allosterically inhibits actin binding, by stabilization of a Fascin-1 conformation in which ABS1 and ABS2 are both distorted (figure 3c).HDX-MS showed that the β-trefoil 4 peptide 398 RKVTGTLDANRSSYDVF 414 , which contacts β-trefoil 1 to form the surface corresponding to ABS1, undergoes a substantial conformational change induced by binding of Nb 3E11 to an adjacent region of Fascin-1 (figure 3b).A comparison of the Fascin-1/Nb 3E11 complex crystal structure to the structure of Fascin-1 alone shows that this disruption of the intramolecular interaction between β-trefoil 4 and β-trefoil 1 in the presence of Nb 3E11 is accompanied by a 25°rotation of β-trefoil 1 so that all the actin-binding residues on β-trefoil 1 have altered positions relative to the remaining portions of ABS1 and ABS2 on the adjacent domains (figures 3d and 5a; electronic supplementary material, movie S1).Thus, the conformational change associated with Nb 3E11 binding to β-trefoil 4 distorts both of the principal actin-binding surfaces of Fascin-1.This allosteric effect, in conjunction with Nb binding directly occluding Lys 379 on β-trefoil 3, explains the inhibitory effect of Nb 3E11 on Fascin-1 actin-bundling activity.

Nb 3E11 disrupts Fascin-1-mediated filopodia
To understand the effect of Nb 3E11 on filopodia, MIA PaCa-2 pancreatic cancer cells were transiently transfected with mCherrytagged Fascin-1 and GFP-tagged Nbs.Images of live cells were acquired using confocal microscopy to avoid fixation artefacts.Cells were also transfected with Nbs alone to check for signal bleed-through between channels (electronic supplementary material, figure S5a).Quantification of the number of filopodia showed that expression of Nb 3E11-GFP led to a significant reduction in the number of Fascin-1-mediated filopodia generated by cells (figure 4a,b).Furthermore, these cells frequently appeared blebby (figure 4a,c).Expressing a higher ratio of Nb 3E11-GFP to mCherry-Fascin-1 in multiple cell lines led to increased, but not significant inhibition of filopodia formation, demonstrating that Nb 3E11 inhibits Fascin-1-dependent filopodia production in cellulo in a dose-dependent manner (figure 4c-e and electronic supplementary material, figure S5b,c).Nb 3E11 had no effect on cell proliferation over the duration of these experiments (electronic supplementary material, figure S6).
In addition to the paucity of filopodia in SK-OV-3 cells expressing Nb 3E11-GFP, the filopodia that remained exhibited significantly shorter lifetimes (figure 4f,g and electronic supplementary material, movie S2).This was not consistently observed in B16-F1 and MIA PaCa-2 cells (electronic supplementary material, figure S5d,e).These data indicate that Nb 3E11 expression reduces Fascin-1-mediated filopodia formation and stability in cells.

Inhibition of Fascin-1 actin-bundling activity
Fascin-1 is a potent driver of metastasis and its overexpression is associated with the most aggressive human carcinomas [17,45].Depletion of Fascin-1 or inhibition of its actin-bundling activity in cancer cells reduces tumour invasion and metastasis [25,46,47].
royalsocietypublishing.org/journal/rsob Open Biol.14: 230376 To date, efforts to inhibit Fascin-1 activity have focused on the development of small-molecule inhibitors targeting the cleft between β-trefoils 1 and 2 [10,[22][23][24].The inhibitors BDP-13176 and NP-G2-029 bind in this cleft, blocking ABS2 and inducing a rotation of β-trefoil 1 that also distorts ABS1 [22,24].The compact arrangement of β-trefoil domains that is observed in crystal structures in the absence of inhibitors is considered to correspond to the actin-bound conformation in which the principal actin-binding surfaces are assembled at the interfaces between β-trefoil 1 and adjacent domains.Interfering with the position of β-trefoil 1 within this arrangement correlates with disruption of actin-bundling activity [22,24].In the present work, we report the discovery of Nb 3E11, which distorted ABS1 and ABS2 through interaction with β-trefoil 4, a previously untargeted site for  royalsocietypublishing.org/journal/rsob Open Biol.14: 230376 the inhibition of Fascin-1 actin-bundling activity, and directly occluded Lys 379 on β-trefoil 3, which has been identified as an important ABS residue [9].Nb 3E11 induced a conformational change in the region of β-trefoil 4 adjacent to β-trefoil 1 that caused β-trefoil 1 to be rotated into an inactive position, similar to the orientation adopted in the presence of small-molecule inhibitors (figure 5a).Nb 3E11 is therefore an example of a β-trefoil 3-4-directed inhibitor of Fascin-1 actin-bundling activity, providing evidence for the potential for new inhibitors to target other sites on Fascin-1 (figure 5b).Although the conformations observed in crystal structures of inhibited or mutant Fascin-1 are substantially different from a conformation competent to bind actin, the protein in solution may also sample more open conformations through interdomain motions.FRET analysis of the conformational dynamics of Fascin-1 and fluorescence recovery after photobleaching (FRAP) analysis of the dynamics of Fascin-1 association with actin [49] suggest that Fascin-1 oscillates between a compact, actin-binding conformation and an open state upon phosphorylation at Ser 39 by PKC (figure 3d).Molecular dynamics simulations of Fascin-1 mutants with impaired actin binding display different conformations and increased protein flexibility compared to WT Fascin-1 [50,51].Furthermore, Fascin-1-dependent actin-bundling in functional filopodia is not characterized by stable crosslinking of actin fibres, but rather is a highly dynamic process involving rapid cycles of actin binding and disassociation.Because conformational and actin-binding dynamics appear to be important for the activity of Fascin-1, the restriction of interdomain movements imposed by binding of Nb 3E11 at an epitope incorporating parts of β-trefoils 2, 3 and 4 may also contribute to its inhibitory activity.
Nb 3E11 was observed to inhibit Fascin-1-dependent filopodia formation in several cell lines in a dose-dependent manner, indicating that the Nb 3E11 : Fascin-1 ratio is pertinent to observation of in cellulo inhibition of Fascin-1 function.The epithelial ovarian adenocarcinoma cell line SK-OV-3 was more sensitive to Nb 3E11-mediated inhibition of Fascin-1 than the neural crest derived B16-F1 (mouse melanoma) and MIA PaCa-2 ( pancreatic carcinoma) cells lines, which both exhibit a mixed and dynamic morphology in culture, suggesting that the cytoskeletal phenotype of the cells may determine the efficacy of Fascin-1 inhibition [52].

Evidence for ABS3 on Fascin-1
An outstanding question regarding the actin-bundling activity of Fascin-1 is whether it possesses a third ABS.Alanine-scanning mutagenesis of Fascin-1 surface residues has identified a patch of residues that are important for actin-bundling but which lay on β-trefoil 3, a considerable distance from either ABS1 or ABS2 [9].Based on cryo-electron tomography data, a model of the Fascin-1 complex with F-actin has been proposed in which Fascin-1 ABS 1 and 2 form a double actin-binding site on one side of the protein and ABS3 provides a single actin-binding site on the opposing side, enabling bundling of two adjacent actin filaments [48,53].Nb 3E11 occludes Lys 379 , which is a key residue within this putative third ABS, and we suggest that this direct mechanism may contribute to the inhibitory effect of Nb 3E11 on Fascin-1 actin-bundling activity.Further work is required to clarify the role of ABS3 and whether it can be exploited in the development of Fascin-1 inhibitors.

Applications of single-domain antibodies
Single-domain antibody (sdAb) technology has the potential to provide targeted inhibitors by an alternative pathway to traditional small-molecule drug discovery.sdAbs are small, soluble, extremely stable antibody domains, they bind to cryptic shallow epitopes that are rarely sampled with high affinity by traditional methods, and they can be isolated in a relatively short time.Indeed, sdAbs can be used directly as biological therapeutics for extracellular targets as exemplified by the success of caplacizumab for the treatment of acquired thrombotic thrombotcytopenic purpura [43].However, like most proteins, sdAbs are too large and hydrophilic to pass readily across cell membranes.Many strategies have been explored to overcome this and facilitate the delivery of sdAbs into the interior of cells in a manner that might work outside the laboratory context: adjustment of surface charge through substitution of residues in the invariant regions for basic amino acids or addition of cell-penetrating tags or other carriers have been shown to enhance cellular uptake [54,55], the robustness of sdAb proteins may make them suitable for intracellular delivery as cargo by lipid nanoparticles [56], and introduction of sdAbs at the nucleic acid level is an emerging therapeutic possibility [57].However, reliable means for their delivery across cell membranes still have to be developed before sdAbs directed against intracellular targets, such as Nb 3E11, can be used directly as therapeutics.Nevertheless, such sdAbs may identify sites of protein-protein interaction or new potential sites of target inhibition by acting as competitor molecules either directly or allosterically in vitro.This potential is royalsocietypublishing.org/journal/rsob Open Biol.14: 230376 demonstrated by Nb 3E11, which efficiently inhibited Fascin-1 actin-bundling activity by binding at a previously untargeted site on Fascin-1.In addition to Nb 3E11, we identified several Nbs which bound to Fascin-1 without altering its actin-bundling activity.Similarly, a previously identified Nb called FASNb5 alters Fascin-1 actin bundle morphology without affecting bundling activity [58,59].Such reagents may be useful for staining in super-resolution imaging methods which benefit from the smaller size of sdAbs compared to IgGs [30,60,61].
In conclusion, we have isolated and characterized Nb 3E11, a potent sdAb inhibitor of Fascin-1 actin-bundling activity and filopodia formation.Nb 3E11 has potential for use as a tool for Fascin-1 inhibition in cells and to guide development of smallmolecule or peptide inhibitors targeting a previously unexploited site on the protein.

Protein expression and purification
Full-length human Fascin-1 WT and mutants were expressed in CodonPlus RIL E. coli BL21(DE3) cells and purified as described previously [22].The protein was stored in 10 mM Tris pH 7.5, 50 mM NaCl.A version engineered to incorporate an N-terminal royalsocietypublishing.org/journal/rsob Open Biol.14: 230376 Avi-tag sequence was co-expressed with pBirAcm (Avidity LLC) in E. coli B834 cells using media supplemented with biotin in order to generate selectively biotinylated material, which was then purified in a similar manner.C-terminally His-tagged Nbs were expressed in the periplasm of E. coli SS320 cells transformed by pBLIP1 and purified by nickel affinity chromatography and SEC on a Superdex 75 X16/600 column as previously described [62].

Library generation and phage display biopanning
Two llamas were inoculated with 300 µg Fascin-1 1-493 at four intervals over a two-month period.RNA was extracted from the PBMC fraction isolated from blood samples taken from each animal.These procedures were carried out by Preclinics GmbH.cDNA synthesis was performed using the IgG-specific primer CALL002 (5 0 ggtacgtgctgttgaactgttcc 3 0 ).Library construction was performed as described previously [62] with the VHH coding regions ligated into the phagemid vector pBLIP1 and used to transform E. coli TG1 (Lucigen) by electroporation.Library rescue and phage display biopanning were performed as described previously [62,63] using biotinyl-Avi-tagged Fascin 1-493 as the immobilized target.To identify clones specific to Fascin-1, small-scale phage-nanobody expressions were carried out and binding was assessed by ELISA [62].Phagemids encoding Nbs that specifically bound Fascin-1 were purified and sent for DNA sequencing (Eurofins Genomics).

Co-precipitation assays
In vitro recombinant protein co-precipitation assays were performed as described previously using Nbs immobilized on Nickel Sepharose as the bait proteins for untagged Fascin-1 [63].

F-actin-bundling assays
F-actin was generated by polymerization of 50 µM rabbit skeletal muscle G-Actin (greater than 95% purity, Cytoskeleton, Inc.) in 5 mM Tris pH 8.0, 0.2 mM CaCl 2 , 0.2 mM ATP (G-Buffer) supplemented with 100 mM KCl for 1 h at 26°C.Fascin-1 protein was diluted to 0.5 µM in G-Buffer + 100 mM KCl and incubated with equimolar concentrations of Nb proteins for 30 min at 26°C, unless otherwise stated.F-actin was then added to a final concentration of 5 µM and the reaction was incubated for a further 30 mins at 26°C.The mixture was centrifuged at 8000g for 20 min at room temperature (RT).The supernatant and pellet were separated, and the pellets were re-suspended in 100 µl of G-Buffer.Supernatant and pellet fractions were mixed with reducing SDS sample buffer and separated by SDS-PAGE on 4-12% Bis-Tris gels.Gels were stained with InstantBlue Protein Stain (Expedeon) and scanned using a Li-Cor Odyssey CLx Scanner.The images were analysed using IMAGESTUDIO software (v.5.2).

ELISA assays
ELISA assays were performed as described previously [73].The HRP-conjugated anti-His-tag antibody (ab1187; Abcam) was used to resolve binding of Nb 3E11.

Hydrogen/deuterium exchange mass spectrometry
Hydrogen/deuterium exchange mass spectrometry (HDX-MS) experiments were carried out using an automated HDX robot (LEAP Technologies) coupled to an M-Class Acquity LC and HDX manager (Waters Ltd) [74].All samples were diluted to 10 µM in equilibration buffer (10 mM potassium phosphate buffer pH 7.0) prior to analysis.30 µl sample was added to 135 µl deuterated buffer (10 mM potassium phosphate buffer pH 7.0) and incubated at 4°C for 0.5, 2, 5 or 10 min.Following the labelling reaction, samples were quenched by adding 50 µl of the labelled solution to 100 µl quench buffer (50 mM potassium phosphate, 0.05% DDM pH 2.2) giving a final quench pH ∼ 2.5.50 µl of quenched sample (ca 24 pmol) were passed through an immobilized ethylene bridged hybrid (BEH) pepsin column (Waters Ltd) at 500 µl min −1 (20°C) and a VanGuard Pre-column Acquity UPLC BEH C18 (1.7 µm, 2.1 mm × 5 mm, Waters Ltd) for 3 min in 0.3% formic acid in water.The resulting peptic peptides were transferred to a C18 column (75 µm × 150 mm, Waters Ltd) and separated by gradient elution of 0-40% MeCN (0.1% v/v formic acid) in H 2 O (0.3% v/v formic acid) over 7 min at 40 µl min −1 .Trapping and gradient elution of peptides were performed at 0°C.The HDX system was interfaced to a Synapt G2Si mass spectrometer (Waters Ltd).HDMSE and dynamic range extension modes (data independent analysis (DIA) coupled with IMS separation) were used to separate peptides prior to CID fragmentation in the transfer cell.HDX data were analysed using PLGS (v3.0.2) and DynamX (v3.0.0) software supplied with the mass spectrometer.Restrictions for identified peptides in DynamX were as follows: minimum intensity, 1000; minimum products per MS/MS spectrum, 5; minimum products per amino acid, 0.3; maximum sequence length, 25; maximum error, 5 ppm; file threshold, 3/3.
Adherent cells were transfected with Lipofectamine 2000 DNA transfection reagent (Invitrogen) according to the manufacturer's instructions.For each 9.5 cm 2 well of adherent cells, 1 µg of DNA was mixed with 5 µl of Lipofectamine 2000 in Opti-MEM reduced serum medium (ThermoFisher) and incubated for 20 min at RT. Cells were washed with Opti-MEM medium and incubated with the DNA-Lipofectamine mixture for 4 h at 37°C with 5% CO 2 .Thereafter, the DNA-Lipofectamine mixture was removed and replaced with complete medium and incubated overnight at 37°C with 5% CO 2 .

Confocal microscopy
Super-resolution confocal microscopy was performed using a Zeiss 880 LSM equipped with Airyscan, a Plan-Apochromat 63x/ NA 1.4 Oil DIC M27 objective and 488 nm and 561 nm laser lines in frame mode with 1.8× or 5× zoom.Live cells were maintained in an environmental chamber at 37°C and 5% CO 2 .Images were acquired using Airyscan and Airyscan Fast modes in Zen Black Software (v2.3 SP1) and Airyscan processed in Zen Blue Software (v.2.3 Desk).Cells were categorized discretely based on the number of filopodia (0-5, 6-10 or greater than 10).Fluorescence intensity quantification was performed in Fiji (ImageJ) using the Plot Profile plugin.
For nanobody dynamics experiments, cells transfected with nanobody-GFP and mCherry-Fascin-1 were imaged as above in Airyscan Fast mode.To minimize phototoxicity, cells were imaged every 5 s for 2.5 min, and filopodia lifetime quantified using the Manual Tracking plugin in Fiji (ImageJ).

Widefield microscopy
To assess cell proliferation, cells were imaged with an Incucyte Zoom or S3 live-cell imaging system (Sartorius) every 1 h for up to 3 days.Cell confluence was quantified using INCUCYTE ZOOM software (v.2018A) or INCUCYTE S3 software (v.2022A).The experiment was repeated at three times to generate biological replicates.

Statistical analysis
Graphs were plotted and statistical analysis was performed with Prism 7 (GraphPad).The null hypothesis in each case was that there was no difference between the control conditions and Nb 3E11 treatment.To determine the appropriate statistical test to royalsocietypublishing.org/journal/rsob Open Biol.14: 230376

Figure 1 .
Figure 1.Identification and characterization of Fascin-1 specific Nbs.(a) Sequence alignment of Fascin-1-specific Nbs.CDR regions are coloured red.Nb sequence regions are classified according to the IMGT standard [44].The N-terminal 'MA' residues and C-terminal His-tag present on each Nb are not shown.(b) Phagenanobody ELISA results for Fascin-1 Nbs.Red bars represent reactions on wells coated with Fascin-1.Blue bars represent negative control reactions where no target was immobilized on wells.Representative of N = 2 independent experiments.(c) SDS-PAGE analysis of co-precipitation assay between Fascin-1 and immobilized Nbs.N = 2 independent experiments.(d,e) SDS-PAGE analysis of F-actin-bundling co-sedimentation assays with Fascin-1 and nanobodies 3G11, 3H7 and 3E11.N = 2 independent experiments.S, supernatant; P, pellet.( f ) Representative SDS-PAGE gel from F-actin-bundling co-sedimentation assay with Fascin-1 and Nb 3E11.F, Fascin-1; S, supernatant; P, pellet.(g) Dose response curve of Fascin-1 F-actin-bundling activity in the presence of Nb 3E11, and control Nbs 1 and 2. Percentage inhibition of F-actin-bundling was quantified by densitometry of SDS-PAGE gels shown in (f ) and electronic supplementary material, figure S2b,c.N = 3 biological replicates per condition.Error bars = standard deviation.

Figure 3 .
Figure 3. Mechanism of Fascin-1 inhibition by Nb 3E11.(a) ELISA binding assays between biotinyl-Fascin-1 (immobilized) and Nb 3E11.Responses were plotted against Nb 3E11 concentration and fitted to a one-site specific binding equation (solid line) in PRISM10 (GraphPad) to calculate binding affinity.N = 3 independent experiments.(b) HDX analysis of the Fascin-1/Nb 3E11 interaction.The amino acid sequences are shown for Nb 3E11 and Fascin-1; Nb 3E11 CDRs and Fascin-1 βtrefoil domains are marked with boxes.Residues that exhibit a substantial change in deuterium exchange upon complex formation are highlighted on the sequences of Fascin-1 and Nb 3E11, and on surface representations of the Fascin-1/3E11 crystal structure; increase in fractional uptake (red); decrease in fractional uptake (blue).Residues not covered in the HDX-MS analysis are shown in bold (black).Representative of N = 3 independent experiments.(c) Surface representations of apo-Fascin-1 (PDB 3P53) and Fascin-1/Nb 3E11 (Nb 3E11 is shown in cartoon representation).Mutation of residues coloured red has a substantial negative effect on Fascin-1 actin-bundling activity.Mutation of residues coloured pink has a moderate effect on Fascin-1 actin-bundling activity.Coloured residues are classified into ABS 1, 2 and 3 [9].(d ) Structural alignment of Fascin-1/Nb 3E11 and apo-Fascin-1 (PDB 3P53) with their β-trefoil 1 domains coloured light blue and light pink, respectively, and key actin-binding residues coloured dark blue and dark pink.The PKC phosphorylation site, Ser 39 , is indicated.
a Values in parentheses are for the highest resolution shell.